{"id":129,"date":"2023-03-09T21:11:57","date_gmt":"2023-03-09T18:11:57","guid":{"rendered":"https:\/\/orbone.com.tr\/en\/?p=129"},"modified":"2023-03-30T18:46:37","modified_gmt":"2023-03-30T15:46:37","slug":"technology","status":"publish","type":"post","link":"https:\/\/orbone.com.tr\/en\/technology\/","title":{"rendered":"Technology"},"content":{"rendered":"\n\n\n\n\n[et_pb_section fb_built=”1″ _builder_version=”4.20.1″ _module_preset=”default” custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.20.1″ _module_preset=”default” custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”]

Orbone\u00ae<\/sup> Cell and Tissue Bank Technology Platform<\/strong><\/p>[\/et_pb_text][et_pb_text _builder_version=”4.20.1″ _module_preset=”default” text_orientation=”justified” global_colors_info=”{}”]

Making a Difference with Quality and Safety<\/strong><\/p>\n

A combination of common methods, chemical solvents, heat and oxidizing agents are used to clean, decellularize and sterilize tissue. In most processes, the use of solvents such as chloroform or acetone is necessary to defeat bone or decellularize tissue. The presence of oil, dermis or cells prevents tissue wetting, thereby reducing the activity of aqueous oxidizers.<\/p>\n

Traditional tissue processing techniques rely heavily on chemical and biological agents. These agents adversely affect the natural, structural and mechanical properties of grafts. Chemicals cannot sufficiently penetrate deep into bone and soft tissue. As a result, the risk of toxic residues and immunological reactions, as well as processing efficiency, is reduced.<\/p>\n

Orbone\u00ae Cell and Tissue Bank’s eCOO\u00ae scCO2 Technology, on the other hand, is the ideal method for cleaning, decellularization, virus inactivation, sterilization and inoculation while preserving the specific properties of the original tissues, depending on the nature of the raw tissue.<\/p>\n

This unique process overcomes the shortcomings of tissues produced by conventional techniques, increasing sterilization, safety and regenerative properties, keeping the patient and patient safety as the highest priority. It accelerates the recovery of the patient and help them to gain their normal life in a very short time.<\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”]

eCOO\u00ae<\/sup> Supercrit Technology<\/strong><\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.20.1″ _module_preset=”default” custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.20.1″ _module_preset=”default” text_orientation=”justified” global_colors_info=”{}”]

Besides viral inactivation, this process additionally removes fats, cell debris and marrow proteins. As the result, the organo-mineral structure of bone tissue which consists of apatite carburized in bone is perfectly preserved.<\/p>\n

Various applications have been developed to date to devitalize bone tissues. These usually contain solvents for oxidative treatment of oils. In traditional methods, solvent (chloroform or acetone) is the main ingredient to dissolve the fat in the marrow cavity that contains adipose fat cells. The presence of large amounts of lipids inhibits aqueous oxidants, reduce the moisturizing ability of the tissues. Since diffusion in bone tissue is limited, the volume of bone fragments can be treated less effectively.<\/p>[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/orbone.com.tr\/en\/wp-content\/uploads\/2023\/03\/orbone-technology-platform-ecoo-supercrit.jpg” alt=”Orbone Technology Platform – eCOO Supercrit” title_text=”Orbone Technology Platform – eCOO Supercrit” url=”https:\/\/orbone.com.tr\/en\/technology” align=”center” _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.20.1″ _module_preset=”default” custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.20.2″ _module_preset=”default” text_orientation=”justified” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]

In addition, the use of degreasing solvents can cause unwanted toxic residues. Orbone\u00ae<\/sup> bone tissues are exposed to high pressure using eCOO\u00ae<\/sup>scCO2. As a result of this process, CO2 comes to “supercritical” phase, a phase between liquid and a gas. Regardless of the volume of the tissues, after transforming into the supercritical state, CO2 shows a solvent character, ensuring the diffusion of lipids from the tissues. Being a natural substance, eCOO\u00ae<\/sup> scCO2 leaves no toxic residue.<\/strong><\/p>\n

Orbone\u00ae’s production technology, eCOO\u00ae scCO2, is licensed and registered by the Dutch Ministry of Health according to European Directives (2004\/23EC, 2006\/86EC, 2006\/17EC).<\/strong>
Orbone\u00ae human cells and tissues are meticulously produced and licensed by the Cell and Tissue Bank by the Turkish Republic Ministry of Health, are produced by applying the quality and safety requirements of the European directives.<\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.20.1″ _module_preset=”default” custom_padding=”||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”]eCOO\u00ae<\/sup> Technology Approach and Advantages<\/strong>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.20.1″ _module_preset=”default” custom_padding=”||0px||false|false” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/orbone.com.tr\/en\/wp-content\/uploads\/2023\/03\/orbone-ecoo-supercrit-technology.jpg” alt=”Orbone eCOO Supercrit Technology” title_text=”Orbone eCOO Supercrit Technology” _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.20.1″ _module_preset=”default” text_orientation=”justified” global_colors_info=”{}”]

Orbone\u00ae eCOO\u00ae scCO2 Technology provides an effective platform for cleaning and sterilizing tissues. Studies have shown that the use of eCOO\u00ae scCO2 minimally affects the structural integrity of tissue grafts compared to conventional processing techniques. eCOO\u00ae scCO2 therapy also removes the medullary tissues responsible for the adverse immune response. In addition to the eCOO\u00ae scCO2 treatment step, the eCOO\u00ae Clean process includes a viral inactivation step.
The combination of these two techniques has been shown to result in a 12-fold reduction in microbial load in processed grafts. Successful removal of cellular and medullary materials and inactivation of viruses is the most important step in providing better quality and cleaner grafts for patient outcomes.<\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.20.1″ _module_preset=”default” text_orientation=”justified” global_colors_info=”{}”]

Non-sterile cell-free tissues after cleaning from cell and lipid residues can also be sterilized using the technology platform’s eCOO\u00ae<\/sup> Ster process. The resulting sterile tissue can be freeze-dried or fresh-frozen. Alternatively, non-sterile cell-free tissue material can be first freeze-dried prior to final sterilization by irradiation technologies.<\/p>\n

One of the most distinctive features of Orbone\u00ae<\/sup> eCOO\u00ae<\/sup> scCO2 Technology is that it can pass through solids and dissolve materials such as a liquid thanks to its gaseous structure. By controlling or regulating the pressure and temperature, the density or solvent strength of eCOO\u00ae<\/sup> scCO2 can be varied to simulate a wide variety of organic solvents. This dissolving power is applied to obtain, extract and sterilize a wide variety of sensitive materials. The low temperature of the process and the stability of CO2 also allow compounds to be removed without damaging or denaturation of allograft tissues.
Due to these properties, the eCOO\u00ae<\/sup> scCO2 Technology can greatly reduce the presence of microorganisms, thereby thoroughly cleansing the tissues, while also meeting the sterility assurance level of 10-6 (SAL6) for all types of microorganisms.<\/p>[\/et_pb_text][et_pb_text _builder_version=”4.20.1″ _module_preset=”default” text_orientation=”justified” global_colors_info=”{}”]

Viral Inactivation and Safety
<\/strong>A viral inactivation study conducted by Institute Pasteur Texcell in Paris in 1995 demonstrated the effectiveness of this process over viral inactivation. The results obtained are superior to the safety level recommended by the European guidelines (6 log reduction versus 14 log reduction). The results have been confirmed by a study by Sanquin Pharmaceutical Services on behalf of eCOO\u00ae Technology.<\/p>\n

Sterilization
<\/strong>When using eCOO\u00ae Technology to introduce and extract bactericidal sterilizers into tissue like peri-acetate acid, the process renders tissue sterile by inactivating a wide range of bacteria, including Bacillus.<\/p>\n

Biogenesis and Osseointegration
<\/strong>Allografts treated with Orbone\u00ae eCOO\u00ae Technology lead to a much faster osseointegration compared to untreated allografts. Treatments based on Orbone\u00ae eCOO\u00ae Technology do not change the biomechanical properties of the tissue.<\/p>\n

Clinical Evidence
<\/strong>The processed Orbone\u00ae eCOO\u00ae grafts have proven to have superior biochemical composition, biocompatibility, tolerance and biofunctional properties compared to grafts processed using conventional methods.<\/p>\n

State-of-the-art tissue products that are clean and sterile (SAL6) without compromising on structural integrity
<\/strong>\u2022 Excellent biocompatibility and advanced regenerative properties
\u2022 Adaptable to meet tissue specific demands
\u2022 It does not leave hazardous material residues<\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.20.1″ _module_preset=”default” custom_padding=”||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”]

Image of a tissue cleaned with eCOO technology<\/strong><\/a><\/span><\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/orbone.com.tr\/en\/wp-content\/uploads\/2023\/03\/orbone-ecoo-technology-bone-graft.jpg” alt=”Orbone Allograft Supercrit Technology” title_text=”Orbone Allograft Supercrit Technology” align=”center” _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.20.2″ _module_preset=”default” text_orientation=”justified” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]

SEM images confirm that eCOO\u00ae scCO2 minimally affects the extracellular matrix while removing medullary tissues.<\/em><\/strong><\/p>\n

The Orbone\u00ae Tissue Bank eCOO\u00ae Technology Platform encompasses cutting-edge technology handles for cleaning, sterilizing and inoculating biological grafts using a breakthrough solution based on supercritical carbon dioxide scCO2. The eCOO\u00ae scCO2 extraction process is highly effective in inactivating viruses and provides superior inactivation when compared to tissues obtained by conventional methods.<\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]\n\n\n\n\n","protected":false},"excerpt":{"rendered":"

Orbone\u00ae bone tissues are exposed to high pressure by using scCO2. This is called \u201csupercritical processing\u201d, where the CO2 comes to a phase between liquid and gas. Regardless of the volume of the tissues, after transforming CO2 into the supercritical state it acts as selective extraction solvent and ensures the extraction of the lipids from the tissues.<\/p>\n","protected":false},"author":1,"featured_media":136,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[20],"tags":[26,137,29,53,32,33,138,126,120,133,66,198,127,139,140,135,136,197],"class_list":["post-129","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-technology","tag-1cc-allograft","tag-allograft","tag-allograft-price","tag-allograft-tissue-transplant","tag-allogreft-vs-otogreft","tag-allotransplantasyon","tag-bone-graft","tag-cancellous-block","tag-cancellous-chips","tag-cortical-cancellous-chips","tag-ecoo-technology","tag-human-tissue-and-bone-bank","tag-orbone-allograft","tag-orbone-greft","tag-orbone-supercritical-technology","tag-scco2","tag-supercritical-technology","tag-tissue-technology","et-has-post-format-content","et_post_format-et-post-format-standard"],"_links":{"self":[{"href":"https:\/\/orbone.com.tr\/en\/wp-json\/wp\/v2\/posts\/129","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/orbone.com.tr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/orbone.com.tr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/orbone.com.tr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/orbone.com.tr\/en\/wp-json\/wp\/v2\/comments?post=129"}],"version-history":[{"count":10,"href":"https:\/\/orbone.com.tr\/en\/wp-json\/wp\/v2\/posts\/129\/revisions"}],"predecessor-version":[{"id":285,"href":"https:\/\/orbone.com.tr\/en\/wp-json\/wp\/v2\/posts\/129\/revisions\/285"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/orbone.com.tr\/en\/wp-json\/wp\/v2\/media\/136"}],"wp:attachment":[{"href":"https:\/\/orbone.com.tr\/en\/wp-json\/wp\/v2\/media?parent=129"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/orbone.com.tr\/en\/wp-json\/wp\/v2\/categories?post=129"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/orbone.com.tr\/en\/wp-json\/wp\/v2\/tags?post=129"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}